Determining Properties of Pero essays

Determining Properties of Pero essays Determining Properties of Peroxidase Enzyme Jeremy Jones and 2:00 Tues. 1406 lab classmates Biology Department, Tarrant County College, Fort Normal function of peroxidase enzyme is to convert hydrogen peroxide to into water and oxygen. Experiments were carried out to check peroxidase activity at different concentration levels, pH levels, temperatures, and check effect of boiling and Peroxidase is a large protein containing hundreds of amino acids and has an iron ion located at its active site. It is easy to obtain and work with, making it an ideal candidate for lab work. The peroxidase reaction can be measured by following the increased oxygen levels. The simplest way to measure oxygen levels is to test with a dye that reacts to chemically active oxygen. The dye will turn from colorless to colored in a dye-coupled reaction. Guaiacol is a dye that turns brown when oxidized. The enzyme, substrate, and dye can be mixed together and immediately placed in a spectrophotometer. The absorbance at 500nm will increase as more color accumulates. Hypotheses: 1. More peroxidase activity will be observed at higher levels of concentration. 2. More peroxidase activity will be observed at mid-level temperatures and mid-pH levels. 3.Boiling will cause complete denaturation of 4. Hydroxylamine will inhibit all peroxidase activity. All experiments carried out as per Biological Investigations, 6th Edition by Warren D. Dolphin. See Lab Topic 6, Determining the Properties of an Enzyme. 1. Peroxidase activity increased steadily with increased concentration. Tubes 6 and 7 with 2 ml of extract actually went off the absorbance scale at 100 2. Temperature was measured from 4 degrees Celsius to 48 degrees Celsius. Optimum results were obtained at ...